FAQ: The enzymes in the kit were not stored at -20°C right away. Will they still work?

Yes, Proteinase K and RNase A are very stable at room temperature. Storage at 20-25°C for several months will not harm them. However, long term storage at -20°C is recommended and will not only maintain optimal performance longer, it will also reduce the incidence of microbial growth in the tubes when they are opened and closed repeatedly.