New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
HomeFAQsWhy is there a 60°C, 5 min heat step at the end of the assembly reaction?
FAQ: Why is there a 60°C, 5 min heat step at the end of the assembly reaction?
The final incubation step at 60°C favors BsaI cutting, in the absence of DNA ligation. Digesting any uncut or cut/religated destination plasmid still present in the assembly reactions reduces background.
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