HomeFAQsMy sample turned cloudy after adding the Monarch RNA Cleanup Binding Buffer and ethanol. Is this normal?
FAQ: My sample turned cloudy after adding the Monarch RNA Cleanup Binding Buffer and ethanol. Is this normal?
When cleaning up large amounts of RNA (>100 μg), some precipitation may occur following the addition of the Monarch RNA Cleanup Binding Buffer and ethanol to the sample. A pellet containing the RNA of interest may form on the side of the column following the first binding spin. To maximize recovery of this RNA, a second elution is recommended. To maximize recovery of this RNA, please use care when performing elution and ensure the nuclease-free water is directly applied to the pellet. A second elution is recommended to ensure recovery is complete.
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