FAQ: Are the alkaline phosphatases active in NEBuffers?

Quick CIP (NEB #M0525), rSAP (NEB #M0371) and CIP (NEB #M0290) are active in all restriction enzyme NEBuffers 1.1, 2.1, 3.1 and CutSmart™ Buffer, and can be added directly to digested DNA. Antarctic Phosphatase (NEB #M0289) has a strict requirement for zinc and has optimal activity at pH 6.0.

Antarctic Phosphatase can be used in NEBuffers 1, 2, 3 or 4 as well as the unique NEBuffers for EcoRI and BamHI only when supplemented with 10X Antarctic Phosphatase Reaction Buffer to a final concentration of 1X.