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HomeFAQsHow much cDNA input can I use for the NEBNext Ultra II FS DNA library preparation
FAQ: How much cDNA input can I use for the NEBNext Ultra II FS DNA library preparation?
100 pg–20 ng purified cDNA. We recommend that the cDNA be in 1X TE (10 mM Tris pH 8.0, 1 mM EDTA); however, 10 mM Tris pH 7.5–8, low EDTA TE or H2O are also acceptable. If the input DNA is less than 26 µl, add TE to a final volume of 26 µl.