HomeFAQsHow much cDNA input can I use for the NEBNext Ultra II FS DNA library preparation
FAQ: How much cDNA input can I use for the NEBNext Ultra II FS DNA library preparation?
100 pg–20 ng purified cDNA. We recommend that the cDNA be in 1X TE (10 mM Tris pH 8.0, 1 mM EDTA); however, 10 mM Tris pH 7.5–8, low EDTA TE or H2O are also acceptable. If the input DNA is less than 26 µl, add TE to a final volume of 26 µl.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.