HomeFAQsWhat are the differences between BsmBI-v2 and its isoschizomer, Esp3I?
FAQ: What are the differences between BsmBI-v2 and its isoschizomer, Esp3I?
BsmBI-v2 is a Type IIS restriction enzyme that requires incubation at 55°C and is supplied with NEBuffer r3.1. Esp3i is an isoschizomer of BsmBI-v2 that requires incubation at 37°C and is supplied with rCutSmart Buffer™ (>215 restriction enzymes are supplied with rCutSmart). Both these enzymes are used in Golden Gate Assembly.