FAQ: What are the differences between BsmBI-v2 and its isoschizomer, Esp3I?

BsmBI-v2 is a Type IIS restriction enzyme that requires incubation at 55°C and is supplied with NEBuffer 3.1. Esp3i is an isoschizomer of BsmBI-v2 that requires incubation at 37°C and is supplied with CutSmart® Buffer (>215 restriction enzymes are supplied with CutSmart). Both these enzymes are used in Golden Gate Assembly.

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Esp3I