FAQ: Can the Monarch Total RNA Miniprep Kit NEB T2010 be used to purify RNA from buccal (cheek) swabs?

Yes. We recommend collecting buccal swabs into Monarch DNA/RNA Protection Reagent (NEB #T2011) and processing samples as described below.
RNA Purification from Buccal Swabs
  1. Collect cells from swab into 1X Monarch DNA/RNA Protection Reagent.
  2. For every 300 μl of DNA/RNA Protection Reagent/Sample mixture, add 15 μl Monarch Proteinase K. Vortex briefly and incubate at room temperature for 30 minutes.
  3. Vorex sample briefly and spin for 2 min (16,000 x g) to pellet debris.  Transfer supernatant to an RNase-free microfuge tube (not included).
  4. Add an equal volume of Monarch RNA Lysis Buffer (NEB #T2012) and vortex briefly. Proceed to Step 1 of Part 2: RNA Binding and Elution in the Product Manual.