FAQ: Is the N-Glycan Sequencing Kit protocol suitable for digestion of glycoproteins?

Although this protocol has been optimized for digestion of antibodies, it can be utilized with various other glycoproteins. Some glycoproteins are not efficiently deglycosylated with Rapid PNGase F. In this case, the glycoprotein can be treated with PNGase F Glycerol -free, Recombinant (NEB# P0709) using denaturing reaction conditions.