FAQ: Can I use T7 endonuclease I for Genome Editing applications?

Yes. T7 endonuclease I can be used to detect indel formation (on-target editing events) in genome editing workflows. The EnGen Mutation Detection Kit provides reagents for detection of on-target genome editing events. The kit contains T7 endonuclease I in an optimized formulation, protocols and includes the PCR reagents and a control substrate.  T7 endonuclease I can also be used as a stand-alone reagent outside of the EnGen Mutation Detection Kit.  Please refer to this protocol as a starting point.