HomeFAQsWhat temperature should I use for my LunaScript® cDNA synthesis reaction?
FAQ: What temperature should I use for my LunaScript® cDNA synthesis reaction?
The thermostable Luna® Reverse Transcriptase supports efficient cDNA synthesis at elevated temperatures compared to MMLV RT. For most targets, cDNA synthesis can be accomplished with a 10-minute incubation at 55°C. For difficult templates, the cDNA synthesis temperature can be increased up to 65°C.