HomeFAQsIs it necessary to purify my DNA/RNA substrate prior to digestion with the Nucleoside Digestion Mix?
FAQ: Is it necessary to purify my DNA/RNA substrate prior to digestion with the Nucleoside Digestion Mix?
As carryover of certain reaction components (e.g., EDTA, detergents, etc) may result in incomplete digestion, it is highly recommended that DNA or RNA substrates be purified (column purification or phenol/chloroform extraction followed by ethanol/isopropanol precipitation) and resuspended in water before digestion with the Nucleoside Digestion Mix.
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