HomeFAQsCan the Nucleoside Digestion Mix be incubated with substrate for >1 hour?
FAQ: Can the Nucleoside Digestion Mix be incubated with substrate for >1 hour?
Yes, to ensure complete digestion, the Nucleoside Digestion Mix can be
incubated with sample for longer time periods. No signal deterioration has been
observed by incubating DNA or RNA samples with the Nucleoside Digestion Mix
for longer durations (overnight) at 37°C. Samples containing a large number of
modifications (particularly modifications at the 2´-position) may benefit from
overnight incubation with the Nucleoside Digestion Mix in order to achieve
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