FAQ: Can OneTaq® Quick-Load® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?

The presence of an archaeal polymerase does not prevent OneTaq Quick-Load DNA Polymerase from using dU-containing DNA as a substrate. OneTaq Quick-Load DNA Polymerase is compatible with USER®-cloning methods and bisulfite-treated DNA. For protocols employing bisulfite-treated DNA that require a hot start enzyme, we recommend OneTaq® Hot Start DNA Polymerase (NEB #M0481).