HomeFAQsWhich strain of Competent E. coli should I use for general cloning?
FAQ: Which strain of Competent E. coli should I use for general cloning?
NEB 5-alpha Competent E. coli (NEB #C2987) are a high efficiency derivative of DH5α™, the industry standard cloning strain. NEB Turbo Competent E. coli (NEB #C2984) and NEB 5-alpha F´Iq Competent e. coli (NEB #C2992) allow potentially toxic genes to be cloned due to tight control of expression by lac Iq and are suitable for blue/white screening. NEB Turbo Competent E. coli (NEB #C2984) bring unmatchable speed to your transformations with visible colonies after just 6.5 hours and plasmid preparation capability after 4 hours. NEB 10-beta Competent e. coli (NEB #C3019) are a derivative of DH10B™ and can be used for transforming large plasmids and BACs. Dam-/dcm- Competent E. coli (NEB #C2925) can be used for methylation-free plasmid growth. NEB Stable Competent E. coli (NEB #C3040) are suitable for cloning and propagation of DNA with repetitive elements as the frequency of recombination is much lower.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Sign in to your NEB account
Any items placed in your cart as a guest will be lost upon sign in or leaving the site.