HomeFAQsWhat if I have already fragmented my mRNA and just need to perform the First Strand Synthesis Reaction?
FAQ: What if I have already fragmented my mRNA and just need to perform the First Strand Synthesis Reaction?
If you are starting from fragmented mRNA, we recommend following the protocol in Chapter 5 of the Ultra II Directional RNA Library Prep Kit (#E7760) for strand-specific RNA-seq workflows. For non-directional RNA-seq workflows, follow the protocol in Chapter 5 of the Ultra II RNA Library Prep Kit (#E7770).
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