New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
Assembly utilizes two simultaneous enzymatic activities in a single reaction;
endonuclease digestion by BsaI and ligation by T4 DNA Ligase. With optimized
buffer components and enzyme ratios, a single reaction containing a destination
plasmid such as pGGA and inserts (PCR amplicons or precloned) will result
in ligation of inserts with complementary cohesive ends and the accumulation
of assembled product as a function of time. The final assembly has no BsaI
recognition sites, rendering the assembly inert to further digestion by BsaI. For
more information, view our online tutorial at www.neb.com/goldengate.