HomeFAQsCan the Golden Gate Assembly reactions be scaled down?
FAQ: Can the Golden Gate Assembly reactions be scaled down?
Except for very complex, multi-insert assembly reactions, the reactions can be
scaled down 2–3 fold if input volumes allow. This can be done by scaling down
the reaction volume and components proportionately, or keeping the 20 µl
reaction volume but using 2–3 fold less of each DNA component. In this latter
case plate a larger amount of outgrowth to compensate.