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HomeFAQsCan I use other competent E. coli strains than NEB 10-beta? Can I use subcloning efficiency cells?
FAQ: Can I use other competent E. coli strains than NEB 10-beta? Can I use subcloning efficiency cells?
Yes, other cell strains can be used, but large assemblies will require strains
known to maintain large plasmid stability. In our experience NEB 10-beta
competent E. coli (High Efficiency; NEB #C3019) is an excellent choice for
Golden Gate Assembly. For smaller assemblies other strains such as NEB
5-alpha Competent E. coli (High Efficiency; NEB #C2987), NEB Stable Competent
E. coli (High Efficiency; NEB #C3040), NEB Turbo Competent E. coli (High
Efficiency, NEB #C2984) or NEB T7 Express Competent E. coli (High Efficiency;
NEB #C2566) can also be used. Subcloning efficiency cells will result in lower
transformation levels and should not be used for multi-component assemblies.
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