FAQ: What is the activity of Thermolabile USER Enzyme in other buffers?

Thermolabile USER Enzyme is 100% active in a variety of buffers, including CutSmart Buffer (NEB #B7204), NEBuffer 1.1 (NEB #B7201 ), T4 DNA Ligase Reaction Buffer (NEB #B0202 ) and TE Buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA). Activity in NEBuffers is as follows:

NEBuffer 1.1 2.1 3.1 CutSmart®
% Activity 100 75 25 100

Additionally, Thermolabile USER Enzyme is 100% active in many polymerase and PCR buffers (see chart below).

Buffer Activity
(Relative to
T4 DNA Ligase Buffer)
Thermopol Reaction Buffer (NEB#B9004) 100%
Q5® Reaction Buffer (NEB#B9027) 100%
Phusion® HF Buffer (NEB#B0518) 50%
GeneAmp® PCR I (Applied Biosystems®) 100%
GeneAmp® PCR II (Applied Biosystems®) 100%
PCR Buffer (Roche) 100%
GoTaq® Buffer (Promega®) 75%
PCR Buffer (Qiagen®) 100%
Pfu Turbo Cx Buffer (Agilent Technologies) 100%
AmpliTaq Gold® 360 Buffer (ThermoFisher®) 100%
Fast Start™ Taq DNA Pol PCR Buffer (Sigma Aldrich) 100%
PCR Buffer (Invitrogen) 100%
PCR Buffer (Sigma Aldrich) 100%
PCR Buffer (Enzymatics) 100%

 

AmpliTaq Gold®, Phusion® and Thermo Fisher® are registered trademarks and property of Thermo Fisher Scientific.
GeneAmp® and Applied Biosystems®are registered trademarks of Applied Biosystems, Inc.
GoTaq® and Promega® are registered trademarks of Promega, Inc.
Qiagen® is a registered trademark of Qiagen, Inc.
Fast Start™ is a trademark of Sigma Aldrich.