Thermolabile USER Enzyme is 100% active in a variety of buffers, including rCutSmart Buffer (NEB #B7204), NEBuffer r1.1 (NEB #B7201 ), T4 DNA Ligase Reaction Buffer (NEB #B0202 ) and TE Buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA). Activity in NEBuffers is as follows:
| NEBuffer | r1.1 | r2.1 | r3.1 | rCutSmart® |
|---|---|---|---|---|
| % Activity | 100 | 75 | 25 | 100 |
Additionally, Thermolabile USER Enzyme is 100% active in many polymerase and PCR buffers (see chart below).
| Buffer | Activity (Relative to T4 DNA Ligase Buffer) |
|---|---|
| Thermopol Reaction Buffer (NEB#B9004) | 100% |
| Q5® Reaction Buffer (NEB#B9027) | 100% |
| Phusion® HF Buffer (NEB#B0518) | 50% |
| GeneAmp® PCR I (Applied Biosystems®) | 100% |
| GeneAmp® PCR II (Applied Biosystems®) | 100% |
| PCR Buffer (Roche) | 100% |
| GoTaq® Buffer (Promega®) | 75% |
| PCR Buffer (Qiagen®) | 100% |
| Pfu Turbo Cx Buffer (Agilent Technologies) | 100% |
| AmpliTaq Gold® 360 Buffer (ThermoFisher®) | 100% |
| Fast Start™ Taq DNA Pol PCR Buffer (Sigma Aldrich) | 100% |
| PCR Buffer (Invitrogen) | 100% |
| PCR Buffer (Sigma Aldrich) | 100% |
| PCR Buffer (Enzymatics) | 100% |
AmpliTaq Gold®, Phusion® and Thermo Fisher® are registered trademarks and property of Thermo Fisher Scientific.
GeneAmp® and Applied Biosystems®are registered trademarks of Applied Biosystems, Inc.
GoTaq® and Promega® are registered trademarks of Promega, Inc.
Qiagen® is a registered trademark of Qiagen, Inc.
Fast Start™ is a trademark of Sigma Aldrich.
