Fourth of July Shipping Notice: Orders placed on Friday, July 1, 2022, will be shipped on Tuesday, July 5, 2022, for delivery on Wednesday, July 6, 2022.
HomeFAQsWill SP6 RNA Polymerase work on an uncut plasmid?
FAQ: Will SP6 RNA Polymerase work on an uncut plasmid?
Yes, though SP6 is an extremely processive enzyme and will continue to transcribe around a circular template multiple times without disassociating. The plasmid can be linearized with restriction enzymes that leave a blunt or 5´ overhang downstream of the DNA of interest, resulting in run-off transcription.