HomeFAQsWill SP6 RNA Polymerase initiate transcription from a single-stranded DNA template?
FAQ: Will SP6 RNA Polymerase initiate transcription from a single-stranded DNA template?
No. The promoter region must be double-stranded for the polymerase to recognize it and initiate transcription. The polymerase then transcribes using the opposite strand as a template in the 5´ to 3´ direction.
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