HomeFAQsDoes transcription with SP6 RNA Polymerase require a primer?
FAQ: Does transcription with SP6 RNA Polymerase require a primer?
No. Unlike DNA polymerases, no primers are needed for the activity of SP6 RNA Polymerase. The SP6 promoter region must be double-stranded in order for the polymerase to recognize the promoter sequence with transcription starting at the final G. The polymerase then transcribes using the opposite strand as the template from 5´ to 3´. Linearized plasmids, PCR products or synthetic DNA oligonucleotides annealed to a short, SP6 promoter-containing oligonucleotide can all serve as templates for in vitro transcription with SP6 RNA Polymerase.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.