Fourth of July Shipping Notice: Orders placed on Friday, July 1, 2022, will be shipped on Tuesday, July 5, 2022, for delivery on Wednesday, July 6, 2022.
HomeFAQsCan aberrant RNA be produced using SP6 RNA polymerase?
FAQ: Can aberrant RNA be produced using SP6 RNA polymerase?
Yes. There have been reports that aberrant RNA is produced when linearizing a plasmid using restriction enzymes that leave 3´ overhangs. To avoid this, the plasmid should be linearized using restriction enzymes that leave a blunt or 5´ overhang just downstream of the DNA of interest.