neb.com is undergoing maintenance beginning Friday, December 9th, at 5pm ET and will last through Saturday, December 10th, at 3pm ET. Online ordering will not be available during this time. We apologize in advance for any inconvenience this may cause.
HomeFAQsCan aberrant RNA be produced using SP6 RNA polymerase?
FAQ: Can aberrant RNA be produced using SP6 RNA polymerase?
Yes. There have been reports that aberrant RNA is produced when linearizing a plasmid using restriction enzymes that leave 3´ overhangs. To avoid this, the plasmid should be linearized using restriction enzymes that leave a blunt or 5´ overhang just downstream of the DNA of interest.