Home FAQs Can aberrant RNA be produced using SP6 RNA polymerase?

FAQ: Can aberrant RNA be produced using SP6 RNA polymerase?

Yes. There have been reports that aberrant RNA is produced when linearizing a plasmid using restriction enzymes that leave 3´ overhangs. To avoid this, the plasmid should be linearized using restriction enzymes that leave a blunt or 5´ overhang just downstream of the DNA of interest.