HomeFAQsShould I include a no Luna® WarmStart® Enyzme Mix control (-RT Control)?
FAQ: Should I include a no Luna® WarmStart® Enyzme Mix control (-RT Control)?
Yes. Amplification in the absence of the reverse transcriptase in RT-qPCR generally indicates there is contaminating DNA in your RNA input. Treating your RNA samples with DNase will remove any DNA prior to performing RT-qPCR.