Can I use cDNA? Does it matter how I make it?

The Luna^® qPCR Master Mix works extremely well with cDNA prepared using commercially available cDNA synthesis kits. While Luna qPCR is compatible with a variety of commonly-used cDNA kits, we recommend using the Protoscript^® II First Strand Synthesis Kit (NEB #E6560). cDNA does not need to be purified before use in Luna qPCR, but should be diluted at least 1:10 into the qPCR reaction.