HomeFAQsWhere are the +1 transcription positions for the SP6 and T7 promoters for in vitro transcription and translation located?
FAQ: Where are the +1 transcription positions for the SP6 and T7 promoters for in vitro transcription and translation located?
The +1 position for the SP6 promoter is located 50 base pairs upstream from the cloning site for transcription of inserts cloned in a CW orientation, while the +1 position for the T7 promoter is located 62 base pairs downstream from the cloning site for transcription of inserts cloned in a CCW orientation.
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