FAQ: Which NEBNext kits are recommended for ChIP-seq library prep?

he NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) or the NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103) are our top recommendations for ChIP-seq library prep, enabling input amounts of >500 pg DNA, with a fast and streamlined workflow.

Our product line retains one legacy product (NEB # E6240) for ChIP-seq that uses the original, standard library prep workflow; it is longer than the Ultra II workflow and requires additional clean-up steps and higher input amounts (10 ng).