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HomeFAQsCan RtcB Ligase be used in other NEBuffers?
FAQ: Can RtcB Ligase be used in other NEBuffers?
While RtcB Ligase is optimally active in the supplied buffer, it is also
active in NEBuffer 3 and 1 at 30% and 10% activity, respectively. Use
of these buffers requires the addition of 0.1 mM GTP and 1 mM MnCl2.