HomeFAQsHow much Endoproteinase LysC do I really need to digest my protein?
FAQ: How much Endoproteinase LysC do I really need to digest my protein?
We recommend LysC : substrate ratio within 1:50-100. However, we have observed significant digestion products using as low as 1:250. A database search revealed 65% sequence coverage for the latter and > 80% coverage for the former.