FAQ: How can I maximize ligation fidelity when using SplintR DNA Ligase?

Increasing reaction temperature has been shown to improve activity and specificity of SplintR DNA Ligase. Incubation at 37° C gives improved results in detection of microRNA. Addition of ET SSB (NEB #M2401S) has also been shown to increase activity and reduce off-target ligation when ligating DNA probes to RNA using SplintR DNA Ligase.

Please see SplintR DNA Ligase product page for references.