FAQ: Does HiFi Taq DNA Ligase have activity on substrates containing RNA?

HiFi Taq DNA Ligase has activity only on nicked DNA substrates with no mismatches or gaps. HiFi Taq DNA Ligase has no measurable activity on RNA or RNA/DNA nicked substrates.
DNA Ligase

High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.