FAQ: Can I use dUTP and UDG for carryover contamination prevention with the WarmStart LAMP Kit (DNA & RNA)?
Yes, the WarmStart LAMP Master Mix is compatible with dUTP/UDG carryover prevention. No dUTP is contained in the mix, but 700 μM dUTP can be spike into the mix without significant effects on LAMP performance. Note that it is important to use Antarctic Thermolabile UDG (NEB #M0372), as the isothermal temperature (65 °C) of the LAMP reaction is insufficient to inactivate E. coli UDG and use of the E. coli form can result in inhibition of LAMP. Simply add 0.02 U/μL Antarctic Thermolabile UDG and set up reactions at room temperature to destroy contaminating LAMP products.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.