FAQ: What is the difference between the discontinued Protein Deglycosylation Mix (NEB# P6039) and the new Protein Deglycosylation Mix II (NEB# P6044)?

The Protein Deglycosylation Mix II (NEB# P6044) is a new and improved reformulation of the recently discontinued Protein Deglycosylation Mix (P6039).  The Protein Deglycosylation Mix II is now made up of five recombinant, and highly pure enzymes that have been optimized for simultaneous deglycosylation of N- and O-linked glycans.  The exact formulation change between the two products is shown in the table below:

Protein Deglycosylation Mix (P6039) discontinued Protein Deglycosylation Mix II (P6044)
PNGase F (Glycerol free)  (P0705) PNGase F (Glycerol free), Recombinant  (P0709)
O-Glycosidase  (P0733) O-Glycosidase  (P0733)
α2-3,6,8 Neuraminidase (P0720) α2-3,6,8,9 Neuraminidase A  (P0722)
β1-4 Galactosidase  (P0730) β1-4 Galactosidase S  (P0745)
β-N-Acetylglucosaminidase  (P0732) β-N-Acetylhexosaminidasef   (P0721)