LAMP is an isothermal approach to nucleic acid amplification (using a single temperature) and does not require the thermal cycling of PCR. This temperature requirement enables use of more portable or less expensive instruments, or even simple incubators or water baths. As it is not limited by a doubling-by-cycle amplification, LAMP generally produces more DNA than PCR in a more rapid incubation time. It is not uncommon to see ug yields in <15 minutes. Additionally enzymes and conditions for LAMP provide a more robust and inhibitor-tolerant amplification system, with detection of DNA or RNA species directly from a variety of crude sample preparations.
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