FAQ: How much exoglycosidase should be used?

The amount of enzyme required varies when different substrates are used. Start with 1-2 µl for 10-50µg of glycoprotein or 1 nmol of oligosaccharide for one hour in a 10-25 µl reaction. If there is still undigested material, let the reaction go overnight. Reference the detailed specificity provided on the data card.