The intercalating fluorescent dye is provided at a 50X concentration. 0.5 μl per 25 μl LAMP reaction is recommended when monitoring reactions on most real time qPCR instruments. In other instruments, further dilution of the dye may be necessary (to a final concentration of 0.1-0.5X). The SYBR®/FAM channel of common real-time fluorimeters can be used to read the dye.
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