FAQ: Can I use β1-3,4 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?

Yes. Although the optimal buffer for β1-3,4 Galactosidase is 1X Glycobuffer 4 (50mM sodium acetate, pH 4.5), we recommend double digesting β1-3,4 Galactosidase with other exoglycosidases using 1X GlycoBuffer 1 (50mM NaAcetate, pH 5.5, 5mM CaCl2).  We recommend using 1X Glycobuffer 2 (50 mM sodium phosphate, pH 7.5) for digestion of β1-3,4 Galactosidase with PNGase F and/or O-Glycosidase.