FAQ: Will T4 PNK (3' phosphate minus) work in CutSmart Buffer?

T4 PNK (3' phosphate minus) will work in CutSmart Buffer, with the addition of ATP and DTT. To see its % functional activity in Cutsmart, and that of other DNA Modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.