FAQ: Will T4 PNK (3' phosphate minus) work in rCutSmart Buffer?

T4 PNK (3' phosphate minus) will work in rCutSmart Buffer, with the addition of ATP and DTT. To see its % functional activity in rCutSmart, and that of other DNA Modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart.