HomeFAQsWill the Low Range ssRNA Ladder run on 10% TBE-Urea and 15% TBE-Urea gels?
FAQ: Will the Low Range ssRNA Ladder run on 10% TBE-Urea and 15% TBE-Urea gels?
Yes. When using denaturing gels of high % polyacrylamide to run the Low Range ssRNA Ladder, you should use lesser amount of ladder than what is recommended in the protocol for running a 6% TBE-Urea. For example, you only need 0.03µL-0.06µL of ladder per lane on a Novex 10% TBE-Urea (10-well) gel or ≤0.03µL of ladder per lane on a Novex 15% TBE-Urea (12-well) gel.