HomeFAQsWhy the bands of the Low Range ssRNA Ladder on my 2% agarose-TBE gel are not resolved well?
FAQ: Why the bands of the Low Range ssRNA Ladder on my 2% agarose-TBE gel are not resolved well?
You must be sure to do the denaturation of the ladder properly. After adding 2µL of Low Range ssRNA Ladder to 38µL of RNA Loading Dye, you must incubate this mixture at 90˚C for 3-5 minutes or at 70˚C for 10 minutes and chill it on ice for 2 minutes before loading.
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