HomeFAQsWhy is my sgRNA not exhibiting 100% in vitro Cas9 cleavage activity?
FAQ: Why is my sgRNA not exhibiting 100% in vitro Cas9 cleavage activity?
We recommend using target prediction websites to help identify optimum sequences for gene targeting through Cas9. Please refer to the Cas9 product page (NEB #M0386 or NEB #M0641 (NLS)) for protocol information. For general information on Genome Editing and planning experiments utilizing NEB products, please see: https://www.neb.com/applications/cloning-and-synthetic-biology/genome-editing