FAQ: The sgRNA yield is low – how can I improve the yield?

First verify that the yield using the control oligo included in the kit is ≥ 15 µg.  It is imperative that the reactions are set up at room temperature in the exact order listed in the protocol.  Also verify that the target-specific oligo was designed as described in the protocol.  If a higher yield is required, a longer incubation (1-2 hours at 37°C) is reasonable.