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HomeFAQsThe sgRNA is not running as a single band on the gel – why is this happening?
FAQ: The sgRNA is not running as a single band on the gel – why is this happening?
We recommend running the RNAs on a TBE-Urea (denaturing) gel under denaturing conditions. The sgRNA is highly structured and if not fully denatured may run as multiple bands on a gel and may not run at the correct size as compared to a ssRNA ladder such as the Low Range ssRNA Ladder (NEB# N0364). If smearing is present, this may indicate contamination with an RNase. We recommend wearing gloves and using nuclease-free tubes and filter tips.