neb.com is undergoing maintenance beginning Friday, December 9th, at 5pm ET and will last through Saturday, December 10th, at 3pm ET. Online ordering will not be available during this time. We apologize in advance for any inconvenience this may cause.
HomeFAQsIs it necessary to treat my sgRNA synthesis reaction with DNaseI?
FAQ: Is it necessary to treat my sgRNA synthesis reaction with DNaseI?
We recommend DNaseI treatment followed by RNA spin column purification to remove the dsDNA template, as well as unincorporated dNTPs and NTPs.