HomeFAQsCan acyclonucleotides be used in Sanger sequencing?
FAQ: Can acyclonucleotides be used in Sanger sequencing?
Acyclonucleotides are terminators that can substitute for ddNTPs in
Sanger sequencing reactions when used with Therminator DNA polymerase (Gardner, A. and Jack, W. (2002) Acyclic and dideoxy terminator
preferences denote divergent sugar recognition by archaeon and Taq DNA
polymerases. Nuc. Acids Res. PMID: 11788725; Gardner, A.F., Joyce , C.M.
and Jack, W.E. (2004) Comparative kinetics of nucleotide analog
incorporation by Vent DNA polymerase. J. Biol. Chem. 279, 11834-11842.