HomeFAQsIs it necessary to phosphatase treat my sgRNAs?
FAQ: Is it necessary to phosphatase treat my sgRNAs?
RNAs transcribed with a bacteriophage polymerase will possess 5’
triphosphates. Phosphatase treatment will remove these triphosphates
leaving a 5’ OH. If treating with phosphatase we recommend treatment
following DNaseI treatment. The enzyme can not be inactivated by heat,
therefore phenol:chloroform extraction is the preferred method for
inactivating the enzyme.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.