HomeFAQsIs it necessary to phosphatase treat my sgRNAs?
FAQ: Is it necessary to phosphatase treat my sgRNAs?
RNAs transcribed with a bacteriophage polymerase will possess 5’
triphosphates. Phosphatase treatment will remove these triphosphates
leaving a 5’ OH. If treating with phosphatase we recommend treatment
following DNaseI treatment. The enzyme can not be inactivated by heat,
therefore phenol:chloroform extraction is the preferred method for
inactivating the enzyme.