HomeFAQsIs it necessary to DNase treat my sgRNA synthesis reaction?
FAQ: Is it necessary to DNase treat my sgRNA synthesis reaction?
We recommend DNAse treating the sgRNA synthesis reaction with 2µl of the
provided DNaseI (RNase-free) for 15 minutes at 37°C followed by spin
column purification. Removal of DNA template, and leftover NTPs and
dNTPs, will result in a more reliable measurement of RNA yield as
instruments that read A260 (such as a traditional UV spectrophotometer
or NanoDrop™) will read both RNA and DNA and will not give a reliable
concentration. It is important to consider how leftover template, dNTPs
and NTPs may affect downstream applications.