FAQ: Does NEB provide plasmids for sgRNA cloning?

NEB does not provide plasmids for sgRNA cloning but we recommend that you visit Addgene if you wish to obtain sgRNA plasmids.

For quickly transcribing sgRNAs without the need for cloning or PCR, we recommend the EnGen sgRNA Synthesis Kit, S. pyogenes (NEB #E3322S).

Alternatively, oligonucleotide templates encoding sgRNA transcribed by a T7 promoter can be used in conjunction with an in vitro transcription kit such as the NEB’s HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB #E2050) to quickly and efficiently produce guide RNAs.