FAQ: Why do I see a DNA smear on an agarose gel after a restriction digest?

Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

Learn more about what causes this common problem, and how NEB's enzymes are QC'd to avoid DNA smearing.

There are several possible reasons why you may see a DNA smear on your agarose gel after a restriction digest. For a discussion on this topic please refer to the video above.