FAQ: Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?

Yes, NEBNext adaptor and primers for Illumina are compatible with NEBNext kits for Illumina DNA, RNA and ChIP-seq library preparation. However, they are not compatible with Illumina small RNA library preparation. For small RNA library preparation for Illumina, please refer to our kits (NEB #E7300, E7580 and E7560), which contain their own adaptor and barcoded primers.